Bekker-Jensen DB, Martínez-Val A, Steigerwald S et al (2020) A compact quadrupole-orbitrap mass spectrometer with FAIMS interface improves proteome coverage in short LC gradients.Meier F, Brunner A-D, Koch S et al (2018) Online parallel accumulation-serial fragmentation (PASEF) with a novel trapped ion mobility mass spectrometer.Hebert AS, Prasad S, Belford MW et al (2018) Comprehensive single-shot proteomics with FAIMS on a hybrid orbitrap mass spectrometer.Pfammatter S, Bonneil E, McManus FP et al (2018) A novel differential ion mobility device expands the depth of proteome coverage and the sensitivity of multiplex proteomic measurements. ![]() Dodds JN, Baker ES (2019) Ion mobility spectrometry: fundamental concepts, instrumentation, applications, and the road ahead.Riley NM, Hebert AS, Coon JJ (2016) Proteomics moves into the fast lane.Aebersold R, Mann M (2016) Mass-spectrometric exploration of proteome structure and function.Larance M, Lamond AI (2015) Multidimensional proteomics for cell biology.Importantly, the protocol can be readily adapted to other gradients and sample types such as modified peptides. Using the EvosepOne liquid chromatography system, we demonstrate expected results for the analysis of a human cancer cell line at a throughput of 60 samples per day, leading to the quantification of about 6000 protein groups with very high reproducibility. We highlight key acquisition parameters and illustrate their optimization for short gradients. We provide a step-by-step protocol for instrument setup, method design, data acquisition and ion mobility-aware, library-based data analysis with Spectronaut. The parallel accumulation-serial fragmentation (PASEF) operation mode positions the mass-selecting quadrupole as a function of the TIMS separation, which allows highly efficient data-independent acquisition schemes (dia-PASEF), but also increases complexity in the method design. Here we describe the use of a trapped ion mobility-quadrupole time-of-flight (TIMS-QTOF) mass spectrometer for high-throughput label-free quantification with data-independent acquisition. His one great achievement is being the father of three amazing children.Ion mobility separation is becoming an integral part in mass spectrometry-based proteomics. He is one of the founders of the FOAM movement (Free Open-Access Medical education) and is co-creator of , the RAGE podcast, the Resuscitology course, and the SMACC conference. He created the ‘Critically Ill Airway’ course and teaches on numerous courses around the world. He coordinates the Alfred ICU’s education and simulation programmes and runs the unit’s education website, INTENSIVE. He is actively involved in in using translational simulation to improve patient care and the design of processes and systems at Alfred Health. He has completed fellowship training in both intensive care medicine and emergency medicine, as well as post-graduate training in biochemistry, clinical toxicology, clinical epidemiology, and health professional education. ![]() He is an internationally recognised Clinician Educator with a passion for helping clinicians learn and for improving the clinical performance of individuals and collectives.Īfter finishing his medical degree at the University of Auckland, he continued post-graduate training in New Zealand as well as Australia’s Northern Territory, Perth and Melbourne. He is on the Board of Directors for the Intensive Care Foundation and is a First Part Examiner for the College of Intensive Care Medicine. He is also a Clinical Adjunct Associate Professor at Monash University. He is a co-founder of the Australia and New Zealand Clinician Educator Network (ANZCEN) and is the Lead for the ANZCEN Clinician Educator Incubator programme.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |